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Evaluation
of I/D polymorphism of ACE in association with development of ischemic stroke
in a North Indian population
Kanika Vasudeva1 , Sulena 2
, Anjana Munshi1*.
1Department of Human Genetics and
Molecular Medicine, Central University of Punjab, Bathinda-151001, India:
2 Guru Gobind Singh Medical College,
Faridkot, Baba Farid University of Health Sciences, Faridkot-151203, India
*Email Id: anjanadurani@yahoo.co.in/
anjana.munshi@cup.edu.in
Blood
sample;
Stroke
Treatment;
Atherosclerosis;
Venipuncture;
Hypertension
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Abstract
Previous studies have demonstrated that stroke has
a strong genetic component. In the current study, we carried out an
investigation to evaluate the angiotensin converting enzyme
insertion/deletion (ACE I/D) variant of the ACE gene with the development
of ischemic stroke and its subtypes. Three hundred ischemic stroke patients
and an equal number of healthy controls were included in this case-control
study. Blood samples were collected and the presence of ACE gene
polymorphism (I/D) was detected by the polymerase chain reaction (PCR)
technique. Ischemic stroke subtypes were classified according to TOAST
(Trial of Org 10172 in Acute Stroke Treatment) classification. The
genotypic distribution and allelic frequencies between patients and controls
were found to be statistically significant (p<0.001). ACE DD genotype and D allele
associated significantly with increased susceptibility to ischemic stroke
in patients from the Malwa region of Punjab. In
addition, D allele associated significantly with large artery
atherosclerosis including both intracranial large artery as well as large
extracranial large artery (p<0.001). Multiple logistic regression analysis with the
potential confounding risk factors and ACE I/D gene variant revealed that
the I/D polymorphism in ACE gene is significantly [p = .015] associated
with ischemic stroke. We find significant association of this polymorphism
with intracranial large artery atherosclerosis and extracranial large
artery atherosclerosis. Our results indicate that the individuals
bearing ID+DD genotype of ACE gene are at greater risk of developing stroke
than II genotype.
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